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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2007 Jul;48(7):3184–3194. doi: 10.1167/iovs.06-1403

Figure 6.

Figure 6

Effect of silencing the MGP gene in human trabecular meshwork cells. Primary human trabecular meshwork cells were transfected by nucleofector electroporation with either MGP siRNA (40 nM and 80 nM final concentrations) or scrambled negative control siRNA (40 nM final). Forty-eight hours after transfection, cells were harvested and homogenized, and aliquots were taken for total RNA, endogenous ALP, and genomic DNA extraction and determination. RNA was reverse transcribed, analyzed, and normalized for MGP expression by real-time TaqMan PCR using probes, as described in Methods. Scrambled negative control siRNA samples are given a value of 100. Top: percentage of remaining transcripts in cells treated with MGP siRNA compared with those treated with the scrambled negative control siRNA. Bottom: normalized endogenous ALP values in MGP and scrambled negative control siRNA-treated samples (n = 3). *P ≤ 0.0003. MGP siRNA silences the MGP gene efficiently in the human trabecular cells. Silencing MGP results in increased endogenous ALP activity.