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. Author manuscript; available in PMC: 2008 Aug 3.
Published in final edited form as: Biochem Biophys Res Commun. 2007 May 30;359(3):563–567. doi: 10.1016/j.bbrc.2007.05.146

Fig. 2.

Fig. 2

Extracts from Aplysia ganglia treated with 5-HT phosphorylate Ap-CREB1a and this ability lasts 24 h. (A) GST alone and GST-RI were expressed in bacteria. The products were analyzed by SDS-PAGE and visualized by Coomassie Brilliant Blue staining. (B) GST or GST-CREB1a incubated with extracts from Aplysia ganglia and γ-32ATP at 30°C for 30 min. The products were analyzed by SDS-PAGE and assessed by autoradiography. Extracts from Aplysia ganglia without 5-HT treatment weakly phosphorylated GST-CREB1a (upper panel, lane 1) but not GST alone (lower panel, lane 1). Aplysia ganglia treated with 5-HT for 2 h phosphorylated GST-CREB1a efficiently (upper panel, lane 2) and persistently phosphorylated GST-CREB1a 2 to 24 h after (upper panel, lane 2 to 6). “RI” indicates the relative intensity of phosphorylated GST-CREB1a.