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Xenografts of 1500 embryonic stem cells + 4 embryonic bladder mesenchymal shells/graft harvested at 42 days. a&b. Gomori’s trichrome, multilayered cuboidal urothelial cells (red) with sub-urothelial connective tissue (blue) were seen. c&d. Immunohistochemical detection of smooth muscle α-actin (brown) denoting smooth muscle fibrils. e&f. Immunohistochemical detection of broad-spectrum uroplakin (brown) denoting mature urothelium.
