Figure 1. In vivo effect of LPS on leukocyte recruitment in the air pouch.

Total number of leukocytes in pouch lavage was determined by flow cytometry, as described in Materials and Methods. LPS elicits leukocyte recruitment in vivo. Sterile PBS (saline), alone, or containing 100 ng/ml LPS was injected into the 6-day murine air pouches of β₂-adrenergic receptor knockout and control female and male animals. After 4 hours, exudates were collected and the total number of leukocytes was enumerated by flow cytometry as described in Materials and Methods. Number within bars indicates n.