Table 1.

Primer and probe sequences employed for PRNP analysis

Primer/Probe	Sequence (5'-3')
136F2 forward primer	GCAGCTGGAGCAGTGGTAGG
171R2 reverse primer	GATGTTGACACAGTCATGCAC
136A2 probe	GGGAAGFGCCAFGAGCAGGC3
154H2 probe	GACCGFTACTAFCATGAAAACATG3
171R6 probe	CAGTGGAFCGGTAFAGTAAC5AGAA3
141C3 probe	GAGCAGGCCTCTFATAC5FTTTGG3

F and 3 represent fluorescein dT and 3' phosphate respectively. Common SNPs within codons 136, 141, 154 and 171 are underlined. 5 represents the universal base 5-nitroindol employed to neutralise polymorphisms within codons 143 and 175.