Table 2.

Probe Tms and acceptance criteria

Probe	Melting peak	Mean Tm	SD	N	Tm Acceptance Criteria
136A2	A136	62.0°C	0.49	2228	60.0°C – 64.0°C
136A2	V136	53.2°C	0.36	214	51.2°C – 55.2°C
136A2	T136	56.9°C	0.15	8	55.2°C – 58.9°C
136A2	AT137	59.5°C	0.19	7	59.0°C – 60.0°C
154H2	H154	56.6°C	0.51	300	54.6°C – 58.7°C
154H2	R154	49.9°C	0.29	2171	47.9°C – 51.9°C
171R6	R171	55.4°C	0.39	1524	53.4°C – 57.4°C
171R6	Q171	49.8°C	0.31	1392	48.4°C – 51.8°C
171R6	QE175	49.7°C	0.21	12	48.4°C – 51.8°C
171R6	L168Q	47.9°C	0.19	5	46.9°C – 48.4°C
171R6	H171	44.8°C	0.27	168	42.8°C – 46.8°C
171R6	K171	41.0°C	0.15	8	39.0°C – 42.8°C
141C3	F141	53.9°C	0.19	39	51.9°C – 55.9°C
141C3	L141	45.0°C	0.14	6	43.0°C – 47.0°C

The mean Tms for each probe melting peak are presented, where N is the number of peaks investigated and SD is the standard deviation. The peak Tms for the major PRNP alleles were obtained from six 384 well plates prepared on different working days, thereby accounting for sample-to-sample and run-to-run sources of variation. Variant alleles exhibiting uncommon genotypes or additional SNPs are italicised. Acceptance criteria for these rare polymorphisms were estimated from the few samples analysed, minimising the potential for miscalling melting peaks.