Table 2.
Sequence-tagged sites (STS) in the subtelomeric region of mouse Chr 4.
| Locus | Accessiona | Forward primer 5′ → 3′ | Reverse primer 5′ → 3′ | Product size (bp)b | Polymorphic B6/129c | Detection methodd |
|---|---|---|---|---|---|---|
| D4Mit33 | MGI92868 | GGAGGTGTCAGGAGCCCT | CTCCTGACTGAGGTACCAAACC | 126 | Yes | SSLP |
| D4Mit190 | MGI92820 | CAGTGAAGCAAAAAGATTCGG | TTCCAGTCTTGGGAATGTATCC | 144 | Yes | SSLP |
| D4Mit42 | MGI92875 | CATGTTTGCCACCCTGAAAC | CCTCACTTAGGCAGGTGACTC | 100 | Yes | SSLP |
| Tas1r1 | AF301161 | GGAGTTGCAGCCTCTACAGC | CAGGAGAGGAAAATGGCAAA | 100 | Yes | SSCP |
| D4Mit254 | MGI100505 | AATACAATTATCATTTGCATTGTGG | TCGTGGTGGACCTTATCTCC | 135 | Yes | SSLP |
| Trp73 | AF138873 | TGAGATCTGGTGCCCTCTCT | GCCTGATCTAGGCTGGAAAA | 229 | Yes | Both |
| D4Mit209 | MGI92840 | CCTTGCTGGTTCTGGCTAAG | CTATCTCCTGAGTTGCTCTGAGC | 122 | Yes | SSLP |
| D4Mit256 | MGI100503 | CTGGAGAGTTAGAATGGGGTACC | ACAGAGGCGCTTCCTAAC | 132 | Yes | SSLP |
| R75150 | R75150 | ACAGGACAAATGCTGGGTTG | GTGGTAAAGAACGCTTGGCT | 217 | Un | NA |
| BG228522 | BE137821 | GCCGATCCTGGTGATGTACT | ACAATGGCTCAAAACCGTTC | 200 | Un | NA |
| M136B1 | AU042805 | TCCTTTATGTCCAACAGCCA | CATGGTCTGTGATGTGACCA | 164 | No | SSCP |
| K01599 | AA062172 | GGAAAAGGGAGTCGCCATA | GAGCCGCCTAACTCTCACAC | 166 | No | SSCP |
| D4Smh6b | X58261 | CTGTAGGCTGCTTTTATCTTTTG | TGCCCCTTCAGCACATGCCA | 102 | Yes | SSLP |
| AA408705 | TC87041 | GCTTCAGAAAATCGAGGCAC | GCATGGGCTATGATAGGTGG | 232 | No | SSCP |
| Ski | U14173 | ATCTCTTGTTTCGTGGTGGG | AGGATGCCCATGACTTTGAG | 173 | No | SSCP |
| 399I12-T7 | AZ052540 | TGGTGGTGTAATACTATTCCTTTG | TCTTTAATTTTTGGCTTTTTGATACA | 102 | No | SSCP |
| Pkcz | NM_008860 | ACACATTAAGCTGACGGACT | CAAACATAAGGACACCCAGT | 164 | Yes | SSCP |
| R74924 | R74924 | AGTGCCACCAACCTGGTAAG | AAGTGCCTGCAGGGATGC | 165 | No | SSCP |
| M134C6 | AI098070 | CAAAACCACATGGTTACCGA | GCCCTATTGCCAAATGACTT | 264 | No | SSCP |
| 438C18-SP6 | AZ077160 | CGGGACCTAAAACTGGACAA | TGGGGACAGTTACCAGGAAG | 254 | Yes | SSCP |
| 472O18-T7 | AZ114562 | CTTTCCATTCTCCACCCTCA | AGGTCCTAGGGAGAGGTCCA | 260 | No | SSCP |
| 399I12-SP6 | AZ052537 | CAGCTGTGTGCATGTTGACC | CATCATGAAGACTCAGGGCA | 106 | No | SSCP |
| 360M12-SP6 | AZ027787 | AATGATGAAGTGTCAGCCTCAG | CAACAGAACTCAAAGCCTGG | 100 | Yes | SSCP |
| 151E4-SP6 | AZ263331 | GACCTTCGGAAGAGCAGTTG | AGTGTGTGTCGCCATATCCA | 223 | No | SSCP |
| 147A15-T7 | AZ273113 | GTGGTTGCTGGGATTTGAAC | CAAGCAACCAAACAACCAAA | 101 | No | SSCP |
| 307E5-SP6 | AQ982924 | GCTAGTTGGGGAACAAACCA | ACTGCAAATGTCCAACTCCA | 149 | No | SSCP |
| Gnb1 | NM_008142 | GGGCATCTGGCAAAGATTTA | AGATAACCTGTGTGCCCGC | 281 | No | SSCP |
| 37D20-T7 | AZ088686 | GAAAACAATCGGGGAGAAGTC | TGAAATTATCACACGCCAGG | 109 | No | SSCP |
| 415A22-SP6 | AZ057087 | GTCCACACCTGGCTTTTGTT | CAGCACTCAGTGAGGTTCCA | 199 | Yes | SSCP |
| 415G24-SP6 | AZ059760 | ATGTAATGGAAGGGCTGCTG | CAGCACTCAGTGAGGTTCCA | 113 | No | SSCP |
| 34M15-SP6 | AZ238889 | CGCTACTTCGCTTTTATCCG | ATGATGACGTACGACGACGA | 150 | No | SSCP |
| Cdc2L2 | NM_00766 | GGCTTCAGCCTCAAGTTCTG | AAAACAACCAAGTTGCCCTG | 101 | No | SSCP |
| 436P10-T7 | NA | CACAGGCCAAGTTGTTGTTG | CAGGGGACCTTCTGAATGAT | 115 | Un | NA |
| D18402 | D18402 | GGAGTTCTCCTACCCTGGCT | GAGGCTCTGAGCAGTGTCAA | 167 | No | SSCP |
| 360M12-T7 | AZ027785 | CGGTCAGGAGTAGTGTGGGT | CAGCAGCTGATATTGAGGCA | 123 | No | SSCP |
| 138D7-T7 | AZ281648 | ACCTCTAGGGTTTACGGGGA | CCTCAGGTAGTGCAAGCTCC | 199 | No | SSCP |
| 151E4-T7 | AZ263335 | GTCCCAAAAGCTAGCACAGG | TCATGAGCCAACCATGTGATT | 240 | No | SSCP |
| 457N22-T7 | AZ089282 | CCGGAGGACCATAAATCTGA | CCTCAAAAACAAGCCTGAGC | 129 | No | SSCP |
| 37D20-SP6 | AZ088681 | GCTAGCCTTGAAGCCAACAC | TGAACAGCATGCTTACCCAG | 122 | No | SSCP |
| 147A15-SP6 | AZ273111 | TCCGGAGGACCATAAATCTG | CACAGTCCCAGTCATTCCCT | 249 | Yes | SSCP |
| D4Xrf497 | AA117049 | GCGAGACGAGTGGGTAGTTC | ACACTGAAACCTCGCTTGCT | 129 | No | SSCP |
| 280G12-T7 | AZ986251 | GGGCTGGGAATTGAACCTAT | TGAATCCCTTACAGCCTTGC | 420 | Yes | SSCP |
| 49O2-T7 | AZ253249 | TCCCTAGAGGCCTGTCTGTC | TCGTCTCGGAGCCTCTTCTA | 169 | Yes | SSCP |
| 34M15-T7 | AZ238896 | GGGTTTATGTGGCAAGCACT | ACTCCATTTGCCTTTTGTGG | 118 | No | SSCP |
| 118E21-SP6 | AZ274152 | GGGGCAGGTGGGTAATAAGT | CAAAAGCCCAACTCCTTGAG | 271 | Yes | SSCP |
| U37351 | U37351 | GTGGCTTGGTGCTATTGACA | GGGGCTATTAAGGCCATTTT | 160 | No | SSCP |
| 338N4-T7 | AQ998700 | AGTTACACAGCTGGGACGA | GCAAGAGCCTAGCAATCCAC | 245 | Yes | SSCP |
| 284D21-SP6 | AQ932576 | ACAGAAATCCCTCATGCGA | TCAGTGTGGACCAGAAAGTCC | 105 | No | SSCP |
| 387F5-SP6 | AQ998225 | CAGTTACACAGCTGGGACGA | GCAAGAGCCTAGCAATCCAC | 245 | Yes | SSCP |
| D4Mon7 | G67778 | CCCACTATGGTCCCAGAGAA | GCGCTGACATCCTCCTATGT | 187 | No | SSLP |
| M134G01 | AI31686 | CACATTAGCCATTGTCCTGG | GGCAGAAAGGAATCAGAAGC | 161 | Yes | SSCP |
| D4Mon4 | G67775 | ACATGCCTGCCTATCTTTGC | GGAACCTGTTTTCCATGGTG | 197 | No | SSLP |
| Dvl | NM_010091 | AAGTTCATGGGCCTCACCACCTGTG | TACTAGCTACCCTTCACATACC | 238 | No | SSLP |
| D4Mon6 | G67777 | TGAGTGTCCTCTGCCTGATG | CCATGGGAGACCAGAAGGTA | 206 | Un | SSLP |
| Tas1r3 | AF311386 | GCAACAGAGTCACAACTGCC | ACACACAGTACCAACCCCGT | 293 | Yes | SSCP |
| D4Mon3 | G67774 | GCTTACGATGGTCGTGAGGT | GCAAGCAACCTGAACATGAA | 188 | No | SSLP |
| D18346 | D18346 | ATGTCCAGGGTAGAGAGCCC | TGTCCGCAGTGTGGAAACTA | 165 | Yes | SSCP |
| D4Xrf215 | F07942 | TTCCAAGCTCACACATCAGC | GTGCTGCTCTGCATTGAGTG | 124 | No | SSCP |
| 350D2-T7 | AZ032147 | TGTAGGGAATGTTTCTGCACC | ACATGGAACAGGATTCTGGC | 295 | Yes | SSCP |
| D4Mon41 | G67754 | TGCATCACTATTAAGCCTCAACC | AAGAATTTGCAAAGACTGTGAGA | 260 | No | Both |
| D4Mon43 | G67755 | GGTGGCTCAAACCATCCATA | GAGGGCAATGAGCAAAATGT | 203 | Yes | SSCP |
| D4Mon44 | G67756 | GGTCCTGTCTCTGGTTCAGG | TAACACCCACATCAGGCAAC | 201 | Yes | Both |
| D4Mon48 | G67757 | CAGCAGGCAAGATGACCTC | GTCCCTCACCAGCCATGTTA | 205 | Yes | Both |
| D4Mon49 | G67758 | AGCCTGGGCTAAGTTGTGTG | TATGGGCCAATGTTGTTCCT | 204 | Yes | Both |
| D4Mon50 | G67759 | ATGGTGGCTCACAACCATCT | AT TTGTCCTCTGATTGCAGC | 193 | Yes | SSCP |
| D4Mon52 | G67760 | ATGCTCAGCCTGCTTTGTTT | GCTGATAGCCCTGGGTTCTA | 198 | No | Both |
| D4Mon53 | G67761 | TGTACGCACAAATTGACTTGC | GAATCCACATTGCAAAGCCTA | 222 | Yes | SSCP |
| D4Mon54 | G67762 | CACAGGCAAATGAAGGGAAG | CCAGACTTCTCCAGCTCTCC | 187 | Yes | Both |
| D4Mon31 | G67747 | CAGATTCTCCAGCTGTCAGG | CTGTGTTTCCGCACCAAGT | 229 | No | SSCP |
| D4Mon56 | G67763 | CCTGTGGTTGACTAGGCAGAA | GCCTGATAGCCTGGAATACA | 406 | Yes | SSCP |
| D4Mon5 | G67776 | ACCTTGTTCCTGGTGTGAGC | TAGCTGGGACGTGGTATGGT | 200 | No | SSLP |
| D4Mon58 | G67764 | TGCACTGACCGTGATAGAGG | CGGTGTAGCTCTGGCTGTCT | 200 | No | SSCP |
| D4Mon59 | G67765 | CATCTCACCAACTCGCACTT | TTTCTGGGAACAAAGAGGCTA | 418 | No | Both |
| D4Mon60 | G67766 | GAACCCAAGTGTTGGGGTAA | TGGAAGCCCATCTGTCTCTT | 222 | Yes | Both |
| D4Mon33 | G67748 | CAGCAGAGGTGATGGGTTCT | TTGTCACACAGTGGTTAAATGC | 203 | No | SSCP |
| D4Mon62 | G67767 | GGTGTGCACCACCATATTCA | GGGAATTATCAGCCAAAAAGC | 201 | Yes | Both |
| D4Mon34 | G67749 | TAGAACCGTGGCTGAGGACT | CCGTAAGATATGAAAGAACTTGGA | 201 | No | SSCP |
| D4Mon63 | G67768 | GCCCAACTGAAAGCTCAACT | GGAAGGGGGATAACAATTGAA | 263 | Yes | Both |
| D4Mon64 | G67769 | TGCTAATTTCAAGCACAGTGAGA | AGCTTGACACCTTGACAGCA | 369 | Yes | SSCP |
| D4Mon36 | G67750 | CCTTCCTCGTCTGAGCTGTT | TTGGGACGTGACCTGAGAAT | 232 | Yes | SSCP |
| D4Mon66 | G67770 | TTCAATTGAGTTTCTCTCCTCTGA | TGCAGGACCAAGAAGTAGGC | 200 | Yes | SSCP |
| D4Mon37 | G67751 | TATGTGTCTGGCCGTTGTTC | GATGTGGGTGCAGGTGAAG | 206 | No | SSCP |
| D4Mon39 | G67752 | GCTGAGCAGCCTCTAGCAA | ACCATGGCTTTTCCCAGTAA | 241 | No | SSCP |
| 139J18-T7 | AZ260477 | ACCACTTGGGGGCTACTTCT | GCTGATCCCCCTGTGATTTA | 232 | Yes | SSCP |
| D4Mon40 | G67753 | CTGTGCCTTTGGTGATCAGA | TGTGGCACTCTACGGCATAA | 261 | No | Both |
| D4Mon1 | G67772 | GCAGTGAGCTGCAGAGTTTG | AGGCCTACCCAAGGACATCT | 201 | Yes | SSCP |
| 417B22-SP6 | AZ061836 | AAACAGGCATGAAACTCAGGA | GGGTATCATTGTCACCTCCA | 116 | No | SSCP |
| 227G4-T7 | N/A | GGAAGCAAATGCTCCACTAAA | TATCCCTAGCCCCTTGTGTG | 243 | No | SSCP |
| D4Mon28 | G67746 | GACCTTTGGAAGAGCAGTCG | TGGCAGCTCACAATGTCTTT | 296 | No | SSLP |
| D4Mon27 | G67745 | TGCCTTTTTCTCACATTGTCTC | TTAGAAGCAGAGGCAGAGGC | 250 | No | SSLP |
| D4Mon26 | G67744 | TCAGACATCTCTGGCCTCCT | TTCACTAAGTTGCCCAGGCT | 160 | No | SSLP |
| 284D21-T7 | AQ977902 | GGTTTGGGAGTGTTAGGCAA | ACTCAGTTGGCCTCTCCTCA | 138 | No | SSCP |
| 280G12-SP6 | AZ001739 | GCCCCATAAAATCCACTCCT | GCTCCGGAAGGCTAGAAGAT | 233 | Yes | SSCP |
| 387F5-T7 | AQ998226 | GCCCCATAAAATCCACTCCT | TTGCCTAACACTCCCAAACC | 214 | Yes | SSCP |
| 49O2-SP6 | AZ253245 | GATAGTCCCTTAGCCAGCCC | GCCATAGCTCCTCACTGCTC | 218 | No | SSCP |
| 338N4-SP6 | AQ998697 | CAGTTTAGCACCCCACCCTA | TCTGCACCTCTGTTCACCTG | 115 | No | SSCP |
| 130A12-SP6 | AZ266745 | CAGTTAGCACCCCACCCTAA | TCTGCACCTCTGTTCACCTG | 114 | Yes | SSCP |
| D4Mon25 | G67743 | GTTTCACATGTTGTGGTGGC | GGGACCTTTGGGATAGCATT | 131 | No | SSLP |
| Tnfrsf4 | NM 011659 | AGGCAGAAAGCAGACAAGGA | CGACAGCACTTGTGACCACT | 138 | Yes | SSCP |
| D4Mon24 | G67742 | CAGCAGCAAATGACCTTTCA | GAGGCAGGCAGATTTCTGAG | 147 | No | SSLP |
| D4Mon22 | G67741 | GTTGGGGCTGCTCATAGAAA | GCTGTGGCTCTCTTGGAGTT | 422 | Yes | Both |
| D4Mon21 | G67740 | CAGTTCTTCCCGAAAACCAC | TTTCTGGGAACTGAGATGGC | 174 | No | Both |
| A1225779 | AI225779 | GCTGTTAGTGAGGTCAGGGC | CGTAGGTGGCACAGTTGAGA | 104 | Yes | SSCP |
| D4Mon20 | G67739 | TTAGCGTTAGGGTGAGGGTG | GGAGACTACGGACTTGTGGC | 150 | No | Both |
| D4Mon19 | G67738 | GGAAGGTAGGGCCTGGTAAT | GCTCCAAGATCTGTGCGATT | 277 | No | Both |
| D4Mon18 | G67737 | GCTCAGCCCCTGAATCAATA | GGGATCTGCCTGTCTTACCA | 111 | No | Both |
| D4Mon17 | G67736 | GCTCAAGGAAGGACACACCT | TGCTCTTAACATTTTGAGCCAT | 201 | No | SSLP |
| D4Mon16 | G67735 | ATCAGACAGCCCACAACCTC | TATGTGCCACCACACCTGTC | 206 | No | SSLP |
| D4Mon68 | G67771 | ACATGTCCACTGTGGCAAAA | TGTCATGAGTTTGAGGCCAG | 246 | No | SSLP |
| D4Mon2 | G67773 | TGATCTTTCCAAACGCATAAGA | AGACACCCTAGGTCCTGCTG | 151 | No | SSLP |
| D4Mon15 | G67734 | TGTTCCTGAGTTCACAACGC | ATTCCCAGCAACTACATGGC | 269 | No | SSLP |
| D4Mon9 | G67779 | GGACAGGTAGCTCACCCAAC | CCAAGAGTCAGCCTTGGAGT | 200 | No | SSLP |
| D4Mon14 | G67733 | TACTTCCCCTTTCCCGAACT | TCCTTGGTGCTTACCCTCAC | 232 | Yes | SSLP |
| D4Mon13 | G67732 | CTAGGGGACTCTGCCAAGTG | CAAGACACCCAGTCCCAACT | 195 | No | SSLP |
| D4Mon12 | G67781 | GCAAGTTTCAGGAGCTAGGG | CCCCAGAACCAGAGACCATA | 166 | Yes | SSLP |
| D4Mon11 | G67780 | AGCCAGGGCTACACAGAGAA | ACCCTGCTACAACGCAGACT | 153 | No | SSLP |
| 118E21-T7 | AZ274183 | CCCAGAACTCCATCCTCAAA | CCCAACCTGTGGTCAGCTAT | 185 | No | SSCP |
| 382A8-SP6 | AZ018016 | AGCAGGCACAGGTCTCTTGT | AAGAACAGGACAGTGGTGGG | 202 | No | SSCP |
| 139J18-T7 | AZ260480 | TCAGTTACCAAGGGTTTCGG | ATAGGTTGTCACAGGCCAGG | 122 | Yes | SSCP |
| Agrn | M92654 | TGTGACTTCCTCTTCCCCAC | TGAGCCACTCCAGATGTCAG | 156 | No | SSCP |
| 28.MMHAP7FLB4.seq | M92657 | CACTAGAGCTGCCACCTTCC | CCCTCAGCACCACTTTTTGT | 162 | No | SSCP |
| K00231 | AA410003 | GTGCTCTGCAGACAAACCAA | GAGCCATTTTGACCCTTAAA | 154 | Yes | SSCP |
| V2r2 | AF053986 | CAATTGAGGAATGGCTACCAA | TGGCTTCATGTCCATTGTGT | 170 | Yes | SSCP |
| 238O5-T7 | NA | TATAAGCAGCCCCTCATTGG | CAGGCCAGACACTGCTTACA | 244 | Yes | SSCP |
| D4Ertd296e | C80036 | AGGCATATTGTATAATAAATTTGTAGT | CCGGATGACTCTACTTGAC | 201 | Yes | SSCP |
a
Accession numbers beginning with MGI are from the Mouse Genome Database and all other Accession numbers are from GenBank.
b
Product size is obtained from B6 genomic DNA.
c
Un = Unknown, not attempted.
d
N/A = not available.
Methods of detection: SSLP = simple sequence length polymorphisms; SSCP = single-strand conformation polymorphisms. Several markers were within known genes, e.g., R74924 (Pkcz); D18402 (Mmp23); U37351 (Pcee) and 28.MMHAP7FLB4.seq (Agrn). The STSs are ordered from the centromeric end to the telomeric end.