Figure 2.

LPS-induced lung injury modulates transgene expression. A total of 100 μg of pCMV-Lux-DTS in 50 μl were administered intratracheally to mice by electroportation (8 pulses of 10-ms duration and a field strength of 200 V/cm). After 24 hours, phosphate-buffered saline (gray bars) or LPS (black bars; 4 mg/kg) was administered to the lungs, or the animals were not treated with anything (naive, white bars). At 1 or 3 days later, lungs were removed and luciferase activity was measured in tissue extracts. Mean luciferase activities (±SEM; n = 5) are shown.