Figure 3.

Electroporation can be used successfully for gene transfer in LPS-injured lungs. Phosphate-buffered saline (gray bars) or LPS (black bars; 4 mg/kg) was administered intratracheally to mice and, 1 day later, 100 μg of pCMV-Lux-DTS in 50 μl were administered by electroportation. The lungs of untreated mice (naive, white bars) were also electroporated with plasmid. At 1 or 2 days after electroporation, luciferase activity was measured in lung extracts (mean ± SEM; n = 5).