Figure 7.

Electroporation-mediated gene transfer of the Na⁺,K⁺-ATPase reduces bronchoalveolar lavage (BAL) cellularity and protein levels. LPS (4 mg/kg) was administered intratracheally to Balb/c mice and, 1 day later, 100 μg of plasmid was delivered to the lungs by electroporation. At 24 or 48 hours later, BAL fluid was collected from lungs and analyzed for protein levels (A) and total cell counts (B) (mean ± SEM; n = 4). Statistical analysis was by one-way analysis of variance followed by Tukey-Kramer post hoc testing. *P < 0.05 compared with levels seen at 48 hours in LPS-injured mice receiving pcDNA3.