Figure 4.

GSNO activation of Akt requires the GSNO membrane transporter, γGT, and is not duplicated by reduced glutathione. (A) Cos 7 cells were transfected as in Figure 1. Cells were preincubated with 100 μM acivicin in the absence or presence of GSNO. Akt kinase activity was assessed as described (n = 7, *P = 0.005). (B) BPAEC and Cos 7 cells were treated with GSNO, with or without acivicin, and HIF-1α protein was detected in whole cell lysate in treated and untreated cells by Western blot analysis using anti–HIF-1α antibody. (C) Akt-transfected Cos 7 cells were treated with 100 μM GSNO or 100 μM GSH and Akt kinase activity assessed (n = 5, *P = 0.003).