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. 2007 May 31;37(3):347–356. doi: 10.1165/rcmb.2006-0176OC

Figure 4.

Figure 4.

Relative transcript levels of R1162X and W1282X CFTR in transduced HeLa (A) and CFBE41o− (B) cells. (A) HeLa cells were grown to confluence, treated with various concentrations of NaBu or control (vehicle media) overnight, then assayed by RT-PCR after RNA isolation. All samples are normalized to endogenous ribosomal (18S) RNA levels, and presented relative to controls. W1282X CFTR mRNA levels under control conditions were 72% of R1162X CFTR expression. *P < 0.05 compared with control; n = 6 per condition (± SEM). (B) CFBE41o- cells were grown on permeable supports at an air–liquid interface before RNA isolation and RT-PCR. Cells were then treated with NaBu (2.5 mM) or control (vehicle) conditions. 18S RNA was used as endogenous control. R1162X CFTR mRNA levels under control conditions were 83% of W1282X expression levels. *P < 0.05, n = 3 per condition (± SEM).