Figure 6.

mRNA expression of (A) IL-4, (B) IL-5, (C) IL-13, (D) IFN-γ, and (E) acidic mammalian chitinase (AMCase) in the lung tissue of ovalbumin (OVA)-sensitized wild-type (C57BL/6) and ob/ob mice challenged with aerosols of either phosphate-buffered saline (PBS) or OVA once per day for 7 consecutive days. Lung tissue was collected 24 hours after the cessation of the final aerosol challenge. mRNA transcript copy number was normalized to β-actin transcript copy number. n = 4–10 mice for each group. Solid bars, wild-type; open bars, ob/ob. *P < 0.05 compared with genotype-matched, PBS-challenged controls.