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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Arch Biochem Biophys. 2006 Nov 2;459(1):59–69. doi: 10.1016/j.abb.2006.10.028

Table 1.

Percent activity following mock labeling reactionsa

Ligand presence Mock labeling reaction conditions
100 mM HEPES pH 7.5 100 mM HEPES pH 7.5, 20 % glycerol 100 mM HEPES pH 7.5, 1 μM CPR-K56Q 100 mM potassium phosphate, pH 7.4
None < 18 72 ± 6 120 ± 10 72 ± 6
+ 200 μM 7HC < 18 100 ± 13 105 ± 18 79 ± 3
a

Experiments were performed in 100 mM potassium phosphate pH 7.4, 20 μM dilauroyl-α-L-phosphatidylcholine, 2 U μL−1 catalase, and 0.04 μg μL−1 superoxide dismutase, with 250 M pNP and a NADPH-regenerating system [2 μU μL−1 glucose 6-phosphate dehydrogenase, 10 mM glucose 6-phosphate, 2 mM MgCl2, 1 mM NADP+]. The final enzyme concentrations were 25 nM CYP2E1, 100 nM CPR-K56Q, and 50 nM cyt b5. The reported values reflect the results from average of 2–3 experiments including the standard deviation from the mean.