Table 2 Antibodies used for immunohistochemistry.
| Antibodies/markers | Clone no. | Isotype | Supplier |
|---|---|---|---|
| Anti‐CD3/T cells | SK7 | Mouse IgG1 | Becton Dickinson, San Jose, CA, USA |
| Anti‐CD4 | SK3 | Mouse IgG2 | Becton Dickinson, San Jose, CA, USA |
| Anti‐CD8 | SK1 | Mouse IgG3 | Becton Dickinson, San Jose, CA, USA |
| Anti‐CD163/activated macrophages | Ber‐MAC3 | Mouse IgG1 | Dako A/S, Glostrup, Denmark |
| Anti‐HLA‐ABC/MHC class I | W6/32 | Mouse IgG2a | Dako A/S, Glostrup, Denmark |
| Anti‐HLA‐DR/MHC class II | L243 | Mouse IgG2a | Becton Dickinson, San Jose, CA, USA |
| Anti‐CD54/ICAM‐1 | 84410 | Mouse IgG1 | Serotec, Oxford, UK |
| Anti‐CD106/VCAM‐1 | 51‐10C9 | Mouse IgG1 | Pharmingen, San Diego, CA, USA |
| Anti‐CD31/endothelial cells | EN4 | Mouse IgG1 | Sanbio, Uden, The Netherlands |
| Anti‐human C5b‐9/MAC | aE11 | Mouse IgG2a | Dako A/S, Glostrup, Denmark |
| anti‐IL1α | 1277‐89‐7 | Mouse IgG1 | AMS Biotechnology Europe Ltd, Abingdon, UK |
| Isotype‐matched irrelevant | DAK‐G01 | Mouse IgG1 | Dako A/S, Glostrup, Denmark |
| Isotype‐matched irrelevant | DAK‐G05 | Mouse IgG2a | Dako A/S, Glostrup, Denmark |
| Secondary antibody (biotinylated) | Lot Q0618 | Horse anti‐mouse IgG | Vector Laboratories, Inc., Burlingame, CA, USA |
| Secondary antibody (biotinylated) for MAC staining | AB2, ChemMate Detection Kit, code No. K5001 | DakoCytomation A/S, Glostrup, Denmark |
CD, cluster of differentiation; EN4, human endothelial 4; HLA‐ABC, human leucocyte antigen; ICAM‐1, intercellular adhesion molecule‐1; IL, interleukin; MAC, membranolytic attack complex; MHC, major histocompatibility complex; VCAM‐1, vascular cell adhesion molecule‐1.