Table 3 Intraindividual variations of molecular expression in muscle tissue analysed by conventional and computerised image analysis.
| Indiv. | T.S. | CD3 | CD163 | MHC‐I* | MHC‐II* | MAC | ICAM‐1 | VCAM‐1 | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cap† | Fibres‡ | |||||||||||
| Cells/mm2 | Cells/mm2 | % pos area | Manual | % pos area | Manual | Manual | Cap/mm2 | % pos area | Cap/mm2 | |||
| 1 | 1 | 7.02 | 116 | 0.21 | + | 3.01 | – | + | + | 3.60 | 0.20 | 0.77 |
| 2 | 14.4 | NA | NA | – | NA | – | + | + | 6.76 | 0.06 | 0.54 | |
| 2 | 1 | 38.8 | 230 | 2.96 | ++++ | 27.8 | + | ++ | +++ | 20.8 | 0.43 | 5.68 |
| 2 | 47.6 | 202 | 3.35 | ++++ | 22.9 | + | + | ++ | 19.3 | 0.24 | 6.02 | |
| 3 | 1 | 8.75 | 51.9 | 0.17 | + | 1.61 | – | ++ | – | 20.6 | 0.32 | 1.34 |
| 2 | 12.5 | NA | NA | + | NA | – | ++ | – | NA | 0.30 | 0.92 | |
| 4 | 1 | 55.9 | 537 | 1.10 | + | 4.49 | – | + | ++ | 7.62 | 0.17 | 10.8 |
| 2 | 48.6 | 429 | 1.35 | + | 10.3 | NA | NA | NA | NA | NA | 3.42 | |
| 5 | 1 | 6.6 | 80.2 | 0.36 | ++++ | 9.20 | – | + | – | 20.3 | 0.34 | 1.73 |
| 2 | 0.11 | 43.6 | 0.22 | ++ | 2.38 | – | + | – | 8.56 | 0.13 | 0.31 | |
Cap, capillaries; CD, cluster of differentiation; ICAM‐1, intercellular adhesion molecule‐1; Indiv., individuals; MAC, membranolytic attack complex; MHC, major histocompatibility complex; NA, not assessed; T.S., tissue samples; VCAM‐1, vascular cell adhesion molecule‐1. *The MHC class I and class II antigen expression on muscle fibres was manually estimated and graded as follows: –, no positively stained fibres; +, 1–20% positively stained fibres; ++, 21–40% positively stained fibres; +++, 41–60% positively stained fibres; ++++, 61–80% positively stained fibres; and +++++, 81–100% positively stained fibres.
†The following grading was used for MAC expression in the capillaries throughout the section: –, no positively expressed capillaries; +, 1–3; ++, 4–10; +++, ⩾11 per 200× field positively expressed capillaries.
‡The following grading was used for MAC expression in necrotic fibres and the surface of morphologically normal fibres throughout the section: –, no positively stained fibres; +, 1 necrotic fibre or a few normal fibres positively stained; ++, 1–3 necrotic fibres and a few/many normal fibres or only a lot of normal fibres positively stained; +++, 1–3 necrotic fibres and many normal fibres positively stained or ⩾3 necrotic fibres and a few/many normal fibres positively stained.