Figure 2.

Definition of the sequence and immunogenicity of the peptide containing the CTL epitope recognized by CS-specific CD8⁺ T cells. (A) Mice immunized once, s.c., with the peptide pool PF1–PF5 in IFA. Immune spleen and lymph node cells, obtained 10 days later, were restimulated in vitro with each of the 10-mer peptides and with the 23-mer P1. (B) Mice immunized s.c. with peptide PF3 in IFA. CD8⁺ immune spleen and lymph node cells restimulated in vitro with PF3 or P1 and the number of IFN-γ- secreting T cells determined.