Figure 3.

CS-specific CD8⁺ spleen T cell response of C3H mice primed and/or boosted with FluPf, VacPf, or PF3. (A) Groups of three mice primed and/or boosted i.n. with 10⁴ pfu of FluPf or 10⁷ pfu of VacPf, injected i.p.. Boosters were given 21 days after priming using the same doses and routes of immunization. The number of specific IFN-γ- secreting CD8⁺ T cells in the spleen was determined by an ELISPOT assay 10 days after the booster. (B) Groups of three mice were primed and/or boosted with the peptide PF3 administered s.c. in IFA. Other mice were primed or boosted with VacPf or FluPf. (Bar = mean number of IFN-γ- producing cells/10⁶ immune spleen T cells ± SE of duplicate cultures.)