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Enhancement of the PF3- specific CD8+ T cell response in the spleen of C3H mice primed s.c. with 120 μg of the lipopeptide (Palm)2-KSS-PF3 and boosted 21 days later with 105 pfu of VacPf i.p., 104 pfu of FluPf i.n., or 80 μg of lipopeptide plus 10 nmol of CSB3, injected s.c. The freshly prepared T cells were incubated with PF3-pulsed irradiated LM1 cells, the number of IFN-γ-secreting T cells was determined. The spleens were removed 10 days after immunization.
