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Lipopeptide (120 μg) priming followed 2 weeks later by boosting with FluPf 5 × 103 pfu of C3H mice resulted in protection, manifested as a delay in vaccinia virus replication in the brain of immunized mice challenged with 103 pfu of VacPf. The number of vaccinia pfu in the brain of challenged mice (immunized and controls) was determined as described in Materials and Methods. The results of two identical experiments are shown.
