Figure 1 Microphotographs of Bodipy‐, Nile red (NR)‐, Oil red O (ORO)‐, and May–Grünwald–Giemsa (MGG)‐stained buffy coats: A1–A4, control subjects; B1–B4, carriers (ABHD5 mutation); C1–C4, patients affected by neutral lipid storage disease without ichthyosis; and D1–D4, Chanarin–Dorfman syndrome patients. Filters were I3 (excitation: 450–490 nm) for NR and Bodipy, TX2 (excitation: 540–580 nm) for ORO, and A4 (excitation: 340–380 nm) for 2‐(4‐amidinophenyl)‐6‐indolecarbamidine (DAPI). Bodipy, NR and ORO fluorescence images were merged with DAPI images by Adobe Photoshop software (v. 7). Scale bar: 10 μm.