Table 2 Antibodies and antigen‐retrieval techniques applied.
Antibody | Dilution | Retrieval | Source |
---|---|---|---|
CD3 | 1:50 | Pressure cooker, aqua destillata, 121°C, 5′ | Dako, Glostrup, Denmark |
CD20 | 1:700 | Microwave oven, citrate buffer pH 6, 800 W, 10′ | Dako, Glostrup, Denmark |
CD34 | 1:25 | Pressure cooker, aqua destillata, 121°C, 5′ | Dako, Glostrup, Denmark |
CD79a | 1:1600 | Pressure cooker, aqua destillata, 121°C, 5′ | NeoMarkers, Fremont, California, USA |
COX2 | 1:100 | Pressure cooker, target retrieval solution S 2368, 121°C, 5′ | Dako Corporation, Carpinteria, California, USA |
Flk‐1 | 1:50 | Microwave oven, citrate buffer pH 6, 800 W, 15′ | NeoMarkers, Fremont, California, USA |
Ki‐67 | 1:50 | Pressure cooker, aqua destillata, 121°C, 5′ | Dako, Carpinteria, California, USA |
p53 (PAb240) | 1:50 | Pressure cooker, aqua destillata, 121°C, 5′ | Dako, Copenhagen, Denmark |
VEGF A | 1:50 | Microwave oven, EDTA buffer pH 8, 800 W, 20′ | NeoMarkers, Fremont, California, USA |
COX2, cyclooxygenase 2; VEGF, vascular endothelial growth factor.