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. 2006 Jun 21;60(5):476–482. doi: 10.1136/jcp.2006.038661

Table 2 Antibodies and antigen‐retrieval techniques applied.

Antibody Dilution Retrieval Source
CD3 1:50 Pressure cooker, aqua destillata, 121°C, 5′ Dako, Glostrup, Denmark
CD20 1:700 Microwave oven, citrate buffer pH 6, 800 W, 10′ Dako, Glostrup, Denmark
CD34 1:25 Pressure cooker, aqua destillata, 121°C, 5′ Dako, Glostrup, Denmark
CD79a 1:1600 Pressure cooker, aqua destillata, 121°C, 5′ NeoMarkers, Fremont, California, USA
COX2 1:100 Pressure cooker, target retrieval solution S 2368, 121°C, 5′ Dako Corporation, Carpinteria, California, USA
Flk‐1 1:50 Microwave oven, citrate buffer pH 6, 800 W, 15′ NeoMarkers, Fremont, California, USA
Ki‐67 1:50 Pressure cooker, aqua destillata, 121°C, 5′ Dako, Carpinteria, California, USA
p53 (PAb240) 1:50 Pressure cooker, aqua destillata, 121°C, 5′ Dako, Copenhagen, Denmark
VEGF A 1:50 Microwave oven, EDTA buffer pH 8, 800 W, 20′ NeoMarkers, Fremont, California, USA

COX2, cyclooxygenase 2; VEGF, vascular endothelial growth factor.