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. 2006 Jun 14;60(5):549–554. doi: 10.1136/jcp.2006.037523

graphic file with name cp37523.f1.jpg

Figure 1 Flow cytometry study with FACScan. Red blood cell (RBC) distribution histograms relating number of events and forward light scatter channel (FSC) distribution (size estimation). In normal, biconcave, ellipsoid RBCs, the FSC distribution (size estimation) is bimodal (16). R1 and R2 (gate determination) are determined manually. M1 and M2 are medians of the gates R1 and R2.