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. 2006 Nov 17;192(2):429–442. doi: 10.1677/JOE-06-0132

Figure 5.

Figure 5

(A) Effect of 17β-estradiol (E2; 1·8–180 nM, 24 h) and dexamethasone (Dex; 0·1 μM, 24 h) on the amount of ANXA1 mRNA in TtT/GF cells measured by quantitative PCR. Values expressed as means±s.e.m., n=3 experiments *P<0·01 versus negative control, ANOVA. (B) Effect of E2 (1·8–180 nM, 24 h) on the amount of ANXA1 protein in TtT/GF cells. Western blot: lanes 1 and 2, control; lanes 3 and 4, 1·8 nM E2-treated; lanes 5 and 6, 18 nM E2-treated; lanes 7 and 8, 180 nM E2-treated. (C) and (D) Integrated densitometry data of the effects of (C) increasing concentrations of E2 (0·18–1800 nM, 24 h) and (D) duration of treatment with 18 nM E2 (3–24 h) on the amount of ANXA1 in TtT/GF cells. Open columns, vehicle control; filled columns, 17β-estradiol-treated. Values expressed as means±s.e.m., n=3 experiments; no significant differences measured versus control, ANOVA.