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. Author manuscript; available in PMC: 2008 Apr 23.
Published in final edited form as: Tetrahedron. 2007 Apr 23;63(17):3575–3584. doi: 10.1016/j.tet.2006.08.110

Figure 4.

Figure 4

(A) General synthetic strategy for the generation of DIS labeled Ψ-site sequences. Green filled circles indicate terminal hydroxyls, red filled circles indicate terminal monophosphates and the ‘x’ indicates the terminal 2′, 3′ cyclic phosphodiester resulting from the ribozyme cleavage reaction. (B) RNA sequence and secondary structure of the DIS stem-loop constructs [DIS40(GA)-ap18 and DIS29(GA)-ap7] designed to form heterodimer complexes. Point mutations in the hexanucleotide sequence [U275 → A275 and C278 → G278] to form the DIS(GA) stem-loop which destroy the palindromic nature of the sequence and promote heterodimer formation are bolded. The position of the 2-AP substitution in the sequence is circled. (C) Sequence and secondary structure of the RNA construct containing the truncated Ψ-site sequence and a 3′ cis-acting hammerhead ribozyme. The sequence positions required for ribozyme activity are shadowed in grey and the cleavage site is indicated by an arrow.