. Author manuscript; available in PMC: 2008 Apr 23.

Published in final edited form as: Tetrahedron. 2007 Apr 23;63(17):3575–3584. doi: 10.1016/j.tet.2006.08.110

Table II.

T4 RNA Ligation Reaction Conditions

2.5 nanomoles	Donor RNA (trunc Ψ-site)
5.0 nanomoles	Acceptor RNA (DIS(GA))
15%	PEG8000
10 mM	MgCl₂
50 mM	Tris-HCl (pH 7.5)
100 μM	ATP
10 μM	Bovine Serume Albumin (BSA)
1.0 mM	Hexammine cobalt chloride (HCC)
1600 Units	T4 RNA ligase