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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Apr 13;1773(7):1124–1132. doi: 10.1016/j.bbamcr.2007.04.003

Figure 3. Nrg1p, Nrg2p, Msn2p and Msn4p in Izh2p-dependent FET3 repression.

Figure 3

For panels A, B, C and F, white bars show strains carrying empty expression vector and black bars show strains carrying Izh2p overexpression vector. For panels D and E, white bars show strains carrying empty expression vector and grey bars show strains carrying Nrg2p-TAP overexpression vector. For all panels except C, activities are shown as a percentage of fully induced activity in the isogenic wild type strain grown in iron-deficient LIM. (A and B) The repression of pFET3-397 lacZ activity by Izh2p overexpression in the BY4742 wild type strain is absent in the nrg1Δ and nrg2Δ mutant strains. Similarly the repression of activity seen in MCY5326 is not seen in the nrg1Δnrg2Δ strain. (C) Overexpression of Izh2p represses a FLO11-lacZ construct in the Σ1278b wild type strain. lacZ activities are shown in Miller Units (D and E) Overexpression of TAP-tagged Nrg2p resulted in repression of pFET3-397 in BY4742 wild type. Similarly Nrg2p-TAP repressed ferroxidase activity in MCY5326 wild type but not in the isogenic nrg1Δnrg2Δ strain. (F) The constitutive repression of ferroxidase activity in low iron medium seen in an msn2Δmsn4Δ strain relative to the MCY5326 isogenic wild type strain can be partially alleviated by concomitant deletion of nrg1Δ and nrg2Δ to generate the quadruple mutant. Izh2p overexpression has no effect on ferroxidase activity in strains lacking Nrg1p/Nrg2p.