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. Author manuscript; available in PMC: 2007 Sep 26.
Published in final edited form as: J Mol Biol. 2006 Oct 3;365(1):196–210. doi: 10.1016/j.jmb.2006.09.084

Table 1.

Monoclonal antibodies mapped using surface displayed BoNT/A domains.

Antibody BoNT/A KD (M) Domain specificity In vitro neutralization (fold over control)
HuC25 4.5 × 10−11 HC 6.82*
3D12 6.1 × 10−11 HC 3.02
S25 1.7 × 10−9 HC 1.85
9D8 1.2 × 10−9 HN ND
ING2 9.6 × 10−12 LC ND
ING1 3.1 × 10−10 LC-HN ND

Solution KD of HuC25, 3D12, ING1, and ING2 were measured in a flow fluorimeter (KinExA) (ref. 22 and unpublished data). KD of S25 and 9D8 were measured using surface plasmon resonance (BIAcore) 25. In vitro neutralization was determined as time to 50% neuroparalysis in the mouse hemidiaphragm assay and is expressed as fold over control 17.

*

In vitro neutralization data for C25, murine precursor of humanized C25, HuC25.