Table 2.
Binding of BoNT/A HC alanine mutants to HuC25 or 3D12 FAB fragments.
| BoNT/A HC mutant | HuC25 FAB binding | BoNT/A HC mutant | 3D12 FAB binding |
|---|---|---|---|
| S896A | ++++ | N1126A | ++++ |
| K897A | ++++ | N1127A | ++++ |
| N899A | ++++ | V1128A | ++++ |
| I900A | ++++ | G1129A | − |
| G901A | +++ | I1130A | − |
| S902A | ++++ | R1131A | − |
| K903A | +++ | K1187A | ++++ |
| N905A | ++++ | ||
| Q915A | ++++ | ||
| F917A | ++++ | ||
| N918A | ++ | ||
| L919A | ++ | ||
| E920A | − | ||
| S921A | ++++ | ||
| E925A | ++++ | ||
| I927A | ++++ | ||
| K929A | ++++ | ||
| N930A | ++++ | ||
| F953A | − | ||
| N954A | ++++ | ||
| I956A | ++++ | ||
| C1060A | ++++ | ||
| R1061A | + | ||
| D1062A | + | ||
| T1063A | + | ||
| H1064A | − | ||
| Y1066A | ++ | ||
| *HCN (876-1092) | ++++ |
++++
binding of Ala mutant similar to wild-type; −, complete loss of binding;
*
Deletion mutant of HC, lacking the C-terminus portion (amino acids 1093–1296) of the domain.