Figure 6. Macrophage ROS suppress IL-2 but not IFN-γ production by Ncf1 mutant T cells.

Purified CD4⁺ T cells from CII immunized Ncf1^*/*MN^– (white bars), Ncf1^*/*MN⁺ (gray bars), and Ncf1^+/+MN^– (black bars) were incubated with purified p-macrophages from naive mice from the same genotypes (depicted on the x axes) in all 9 combinations and restimulated with lathCII (A), PPD (B), or nothing (Ctr) (C). IFN-γ production, IL-2 production, and the proliferative response were determined. IFN-γ production was increased in all conditions with Ncf1^*/*MN^– T cells whereas IL-2 production and proliferation were suppressed by macrophages originating from Ncf1^*/*MN⁺ or Ncf1^+/+MN^– mice. Means ± SEM of 6-8 mice per group obtained from 3 different experiments are shown. ^#P < 0.05, ^##P < 0.01.