Table 1.

Various parameters of control vs oral BaP-treated mice of the five genotypes under study

Genotype:	Cyp1(+/+) wild-type	Cyp1a1(−/−)	Cyp1a2(−/−)	Cyp1a1/1a2(−/−)	hCYP1A1_1A2_Cyp1a1/1a2(−/−)
Clinical appearance	Healthy	Sickly	Healthy	Sickly	Healthy
Blood [BaP] (ng/ml) after 5 days	1.3 ± 0.7	*29 ± 19	†0.51 ± 0.20	*62 ± 5.2	†0.80 ± 0.10
Liver size (g)/body weight (g)	4.3 ± 0.28	*5.9 ± 0.18	†4.2 ± 0.65	4.5 ± 0.31	*6.0 ± 0.31
Spleen size (g)/body weight (g)	0.26 ± 0.01	*0.11 ± 0.01	†0.27 ± 0.02	*0.11 ± 0.01	†0.21 ± 0.03
Thymus size (g)/body weight (g)	0.14 ± 0.01	*0.03 ± 0.002	†0.13 ± 0.2	*0.03 ± 0.02	*,†0.07 ± 0.006
Plasma ALT (U/Liter)	75 ± 25	*390 ± 130	†60 ± 10	*240 ± 23	†53 ± 9.3
Plasma AST (U/Liter)	140 ± 12	*290 ± 91	†130 ± 20	*360 ± 77	*,†180 ± 19
% lymphocytes in peripheral blood	93 ± 3.0	*62 ± 1.1	†94 ± 3.6	*54 ± 18	†81 ± 7.3

Except for whole blood BaP levels after 5 days, all other parameters were determined after 18 days of oral BaP (125 mg/kg/day). Groups of three or four male mice of the same age (5 weeks) for each genotype were studied. Percent wet weight (g) of liver, spleen, and thymus was measured per gram of total body weight (g). Values are given as means ± SEM.

^*P value <0.05, relative to the Cyp1(+/+) wild-type.

^†P value <0.05, relative to the Cyp1a1(−/−) knockout mouse; all parameters of Cyp1a1(−/−) mice were significantly different from those of Cyp1(+/+) mice.