Table 2.
Expression of nitrate assimilation genes under different N conditions
| Genes | Locus | Fold limiting | Fold stress | Fold 2 hr | Fold 24 hr | |
| Nitrate transport | ||||||
| Low-affinity nitrate transporter | NRT1.1 | At1g12110 | 1.3 | 1.8 | 1.8 | -1.3 |
| NRT1.2 | At1g69850 | - | - | - | - | |
| NRT1.3 | At3g21670 | -1.1 | -2.1 | 1.0 | 1.6 | |
| NRT1.4 | At2g26690 | -1.1 | -2.1 | 1.2 | 2.4* | |
| High-affinity nitrate transporter | NRT2.1 | At1g08090 | - | - | - | - |
| NRT2.2 | At1g08100 | - | - | - | - | |
| NRT2.3 | At5g60780 | - | - | - | - | |
| NRT2.4 | At5g60770 | - | - | - | - | |
| NRT2.5 | At1g12940 | - | - | - | - | |
| NRT2.6 | At3g45060 | - | - | - | - | |
| NRT2.7 | At5g14570 | - | - | - | - | |
| Nitrate reduction | ||||||
| Nitrate reductase 1 (NR1) | At1g77760 | -1.2 | -1.9 | 11.1* | 1.5 | |
| Nitrate reductase 2 (NR2) | At1g37130 | -1.2 | -3.3* | 8.1* | 2.9* | |
| Nitrite reductase (NiR) | At2g15620 | -1.2 | -4.4* | 15.1* | 3.2* | |
| urophorphyrin III methylase (UPM1) | At5g40850 | 1.0 | -1.5 | 10.1* | 1.5 | |
| Fd NADP (+) reductase (FNR) | At4g05390 | 1.3 | 1.2 | 3.6* | 1.0 | |
| At1g30510 | 1.1 | 1.1 | 3.1* | 1.0 | ||
| At4g32360 | - | - | - | - | ||
| At5g66190 | - | - | - | - | ||
| At1g20020 | - | - | - | - | ||
| At5g66810 | - | - | - | - | ||
| Ammonia assimilation | ||||||
| Glutamine synthetase | GS1-1 | At5g37600 | 1.7 | 4.7* | -1.6 | -3.4* |
| GS1-2 | At1g66200 | - | - | - | - | |
| GS1-3 | At3g17820 | - | - | - | - | |
| GS1-4 | At5g16570 | 1.7 | 6.9* | -2.0 | -3.7* | |
| GS1-5 | At1g48470 | - | - | - | - | |
| Glutamate synthetase | GS2 | At5g35630 | - | - | - | - |
| GOGAT1 | At5g04140 | - | - | - | - | |
| GOGAT2 | At2g41220 | - | - | - | - | |
| GOGAT3 | At5g53460 | - | - | - | - | |
| Asparagines synthetase | AS1 | At3g47340 | - | - | - | - |
| AS2 | At5g65010 | -1.3 | -3.2* | 7.6* | 2.9* | |
| AS3 | At5g10240 | - | - | - | - | |
Fold changes under different N conditions were presented: sufficient N vs. limiting N (fold, limiting); sufficient N vs. stress N (fold, stress); stress N vs. 2 hr induction (fold, 2 hr); stress N vs. 24 hr induction (fold, 24 hr). Negative number means gene expression down-regulated in said condition, and positive number means gene expression up-regulated in said condition. *significant from our SAM analysis;
---- no fluctuation under all N conditions