Table 3.
Functional classification | Locus | Gene identification | Fold limiting | Fold stress | Fold 2 hr | Fold 24 hr |
nitrate accumulation | At5g40890 | anion channel protein | ns | -6.8 | 2.9 | 6.0 |
photosynthesis | At1g03600 | photosystem II family protein | ns | -2.9 | ns | 2.3 |
At4g28660 | photosystem II protein W | ns | -2.7 | ns | 3.2 | |
pentose-phosphate pathway | At5g35790 | glucose-6-phosphate 1-dehydrogenase | ns | -2.6 | ns | 2.3 |
At1g24280 | glucose-6-phosphate 1-dehydrogenase | ns | ns | 5.2 | ns | |
starch synthesis | At4g39210 | ADP-glucose pyrophosphorylase | ns | 3.9 | ns | -4.1 |
At2g21590 | ADP-glucose pyrophosphorylase | ns | 2.8 | ns | -3.1 | |
chlorophyll synthesis | At1g03630 | protochlorophyllide reductase | ns | -2.9 | ns | 2.8 |
At4g27440 | protochlorophyllide reductase | ns | -2.9 | ns | 2.3 | |
At5g08280 | hydroxymethylbilane synthase | ns | -2.6 | ns | 2.7 | |
protein degradation | At5g45890 | senescence-specific cysteine protease SAG12 | ns | 7.7 | ns | -4.3 |
nitrogen detoxification | At5g22300 | nitrilase 4 | 2.8 | 11.5 | ns | -4.4 |
anthocyanin synthesis | At4g22880 | leucoanthocyanidin dioxygenase | 2.1 | 14.4 | ns | -7.8 |
At5g42800 | dihydroflavonol 4-reductase | 3.3 | 18.7 | ns | -8.3 | |
At5g13930 | chalcone synthase | ns | 4.1 | ns | -3.4 | |
At1g56650 | Myb transcription factor PAP1 | ns | 6.3 | -3.0 | -6.3 | |
At1g66390 | Myb transcription factor PAP2 | 3.5 | 26.6 | -6.7 | -16.7 | |
phenylpropanoid synthesis | At2g37040 | phenylalanine ammonia lyase | ns | 4.1 | ns | -3.2 |
response to cytokinin | At3g63110 | cytokinin synthase | ns | ns | 3.6 | ns |
At3g48100 | transcription repressor | ns | -4.3 | 3.7 | 3.6 | |
At2g18300 | transcription factor | ns | -8.4 | 7.9 | 6.8 | |
transcription | At1g30500 | CCAAT transcription factor | ns | 4.3 | ns | -4.3 |
At2g34720 | CCAAT transcription factor | ns | 3.4 | ns | -3.0 | |
At3g05690 | CCAAT transcription factor | ns | 3.8 | ns | ns | |
At5g47220 | ATERF2 transcription factor | 2.0 | 2.4 | ns | ns |
Fold changes under different N conditions were presented: sufficient N vs. limiting N (fold, limiting); sufficient N vs. stress N (fold, stress); stress N vs. 2 hr induction (fold, 2 hr); stress N vs. 24 hr induction (fold, 24 hr). Negative number means gene expression down-regulated in said condition, and positive number means gene expression up-regulated in said condition. ns – not significant from our SAM analysis