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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: DNA Repair (Amst). 2007 May 30;6(9):1371–1379. doi: 10.1016/j.dnarep.2007.04.003

Figure 2.

Figure 2

Long-range quantitative PCR analyses of UV-induced lesion removal in HPRT and β-globin genes. (A) Long-range QPCR analysis of lesion removal in the HPRT gene locus for both HMGA1 ON (top panel) and HMGA1 OFF cells (middle and bottom panels). Both ethidium bromide-stained agarose gel and phosphorimage analysis are included for the HMGA1 OFF cell line. Briefly, long-range QPCR products were electrophoresed in 0.8% agarose and either visualized using ethidium bromide (top and middle panel), or, in the bottom panel, dried and exposed to a phosphor-storage screen for detection of [32P]-α-dATP incorporated into the amplified DNA fragments. Lanes 1 and 2 contain, respectively, 50% (10 ng) and 100% (20 ng) of non-UV-treated genomic DNA as controls. (B) Long-range QPCR analysis of lesion removal in the β-globin gene locus for both HMAG1 ON (top panel) and HMGA1 OFF cells (bottom panel). Both panels are representative of ethidium bromide-stained agarose gels. As in panel A, lanes 1 and 2 contain 50% and 100% non-UV-treated genomic DNA as a control.