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. 2006 Sep 20;91(4):459–464. doi: 10.1136/bjo.2006.103895

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Figure 2 Microscopic assessment of limbal‐derived epithelial cells on contact lenses. Primary ocular surface epithelial cells were grown from freshly resected (A, conjunctiva; B, pterygium; C–H, limbal) tissue from patients (A–D, patient 1 and E–H, patient 2) undergoing pterygium surgery, and placed in six‐well tissue culture plates (A and B) or on lotrafilcon A contact lenses (C–H). Phase contrast microscopy showed the presence of and the proliferative capacity of the different ocular surface epithelial cells migrating from tissue explants (A–D, exp) as early as 4 days in culture. The area encompassed by the rectangle in panel C (×40) is magnified in panel D (×100). Cell morphology was also assessed by SEM (E, F) and TEM (G, H). Similar cellular appearance was observed with tissue from at least two other patients (not shown).