Table 1 Primary antibodies used for immunohistochemistry.

Antibody	Source	Catalogue no	Clone	DF	Specificity
Keratin 3/12	USB	C9097‐34M	2Q1040	1:50	Corneal‐type epithelium*
65K keratin†	ICN	69–143	AE5	1:50	Corneal‐type epithelium*
Keratin 15	Biocare	CM068B	LHK15	1:50	Basal stratified epithelium‡
Keratin 19	USB	C9097‐24B	4A36	1:100	Basal limbal epithelium‡, §, glandular‐type epithelium
Pan keratin	Dako	M0821	MNF116	1:50	Keratins 5, 6, 8, 17, 19
p63	Santa Cruz	sc‐8431	4A4	1:50	Proliferative capacity
IgG2a	Dako	X0943	–	1:50	Non‐specific
IgG1	Dako	LS191	–	1:50	Non‐specific

DF, dilution factor.

USB, United States Biological, Swampscott, Massachusetts, USA; CN Biomedicals, Aurora, Ohio, USA; Biocare Medical, Concord, California, USA; Dako Corporation, Carpinteria, California, USA; Santa Cruz Biotechnology, Santa Cruz, California, USA.

*Both antibodies are considered markers of corneal differentiation.

†This antibody was originally purchased from ICN,²⁸ now known as MP Biomedicals, Solon, OH.

‡Antibodies used to identify epithelial cells of progenitor cell phenotype.

§Antibodies used to identify epithelial cells of conjunctival type.