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. Author manuscript; available in PMC: 2008 Aug 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2007 May 18;223(1):10–19. doi: 10.1016/j.taap.2007.05.002

Fig. 9.

Fig. 9

Effect of GSH-EE and PPARα knock-down on Wy14,643-mediated enhancement of cyanide toxicity. Cells were transfected with PPARα-RNAi and 24 h later treated with Wy14,643 (100 μM) for 12 h, followed by KCN (400 μM) for 24 h. In GSH-EE group, cells were loaded with GSH-EE (2 mM) for 1 h and then Wy14,643 (100 μM) for 12 h, followed by KCN for 24 h. (A) Magnification is 200X for phase contrast micrographs of cells: a. KCN; b. Wy14,643+KCN; c. GSH-EE+KCN; d. GSH-EE+Wy14,643+KCN; e. PPARα-RNAi+KCN; f. PPARα-RNAi+Wy14,643+KCN; g. GSH-EE+PPARα-RNAi+KCN; h. GSH-EE+PPARα-RNAi+Wy14,643+KCN. (B) Viability of cells was assessed by MTT assay. Values were expressed as percent viability relative to vehicle-treated control cultures. *Significantly different from control group. #Significantly different from Wy14,643+KCN group. &Significantly different form Wy14,643+GSH-EE+PPARα-RNAi+KCN group. P<0.05.