. Author manuscript; available in PMC: 2008 Aug 15.

Published in final edited form as: Arch Biochem Biophys. 2007 Jun 8;464(2):187–196. doi: 10.1016/j.abb.2007.05.019

Table 2.

Effect of alcohol dehydrogenase, aldehyde dehydrogenase, and P450 inhibitors on recombinant P4503A4 activity.

Inhibitor (100 μM)	% of control activity
Control	100
miconazole	15
troleandomycin	3
cyanamide	92
benomyl	37
4-methylpyrazole	2

The activity of P4503A4 was measured at 25° C using the 7-benzyloxyresorufin as substrate in the presence of 0.5 mM NADPH and E. coli membranes expressing both P4503A4 and NADPH:P450 oxidoreductase (Materials and Methods). The activities were the mean of three assays and SD < 5-8% of the average value. The control activity was 78 pmol/min/nmol P450.