Table 2.

Sequences designed on flexible backbones that were chosen for experimental characterization.

	Sequences*	energy on NM bb (kcal/mol)	energy on x-ray bb (kcal/mol)
Bim	LRPEIRIAQELRRIGDEFNETYTRRV		0
BimL11F	---E--IA--FR-IGD-FN--Y----	−4.4†	6.6
BimD16K	---E--IA--LR-IGK-FN--Y----	2.2†	6.7
X1	---N--IA--MV-IAR-FH--H----		−2.9
X2	---V--VG--MM-IGR-FF--H----		−2.1
N1	---F--VA--LM-FAH-FD--H----	−14.3	8.3
N2	---V--VA--LM-MGK-FY--M----	−6.7	23.0
I1	---H--IV--FK-FGN-IQ--K----	−10.4	30.8
I2	---F--IA--FM-FAQ-MY--I----	−4.5	26.0
I3	---L--LI--LQ-LGY-FN--A----	−2.6	29.1
Ip1	---A--LA--MR-FAR-FE--A----	−10.7	2.2
Ip2	---R--VA--LE-MAN-LR--N----	−10.3	15.1
Ip3	---I--AA--LQ-FAM-FR--D----	−10.1	13.7
Ip4	---M--IA--LR-FAR-FR--D----	−9.8	7.3
N2-Y19N	---V--VA--LM-MGK-FN--M----	−0.1†	9.5
I3-I8A	---L--LA--LQ-LGY-FN--A----	3.3†	3.3
N3	---V--VG--LM-IAR-FD--T----	−7.8	7.6
N4	---F--VA--LN-IAK-FH--F----	−2.7	4.1

All energies are relative to the energy of the x-ray structure of Bcl-x_L/Bim. Dashes indicate the native Bim residue at that position

^*The first Leu was mutated to Glu in experimental tests for all sequences except Bim, BimL11F and BimD16K, see text.

^†Sequences for point mutants were evaluated on all backbones. The lowest energy is reported.