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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: J Struct Biol. 2007 May 16;159(2):166–178. doi: 10.1016/j.jsb.2007.05.001

Figure 4. Xanthine affinity chromatography shows full-length A2aR is active in ligand-binding.

Figure 4

Silver-stained 12% SDS-PAGE gel tracks purification of an IMAC purified population of A2aGFPHis10 through xanthine ligand affinity chromatography. The IMAC elute lane denotes protein loaded onto the XAC column which has been desalted and diluted in purification buffer containing 0.1% DDM/0.1% CHAPS/0.02% CHS as described in Materials and Methods. XAC wash lanes represent undiluted samples from 4 mL fractions collected as unbound material from the column. XAC elute lanes represent undiluted samples collected from 4 mL fractions and show active A2aGFPHis10 that was eluted from the column with 20mM theophylline.