A, C, E, G) Quantification of total Fos expression in the dorsal horn of nondiabetic and diabetic mice. Comparisons between nondiabetic and diabetic mice revealed a significant decrease in Fos-positive neurons at weeks 5 and 7.
B, D, F, H) Quantification of Fos expression within laminar regions. B) One week post-STZ, an analysis of Fos-positive neurons within specific laminar regions revealed no significant differences between nondiabetic and diabetic animals. C) In contrast, analysis at 3 weeks post-STZ revealed a significant reduction in Fos-positive neurons within laminae I/II in diabetic versus nondiabetic mice. D) More severe reductions in Fos-positive neurons in diabetic mice were evident at 5 (D) and 7 (E) weeks post-STZ in all laminae, with the exception of laminae V/VI at 7 weeks. Data plotted as means +/− standard error of mean. * P < 0.05 vs nondiabetic mice.