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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2007 Jan 8;42(7):985–992. doi: 10.1016/j.freeradbiomed.2006.12.026

Fig. 7.

Fig. 7

Representative kinetic traces of ABTS (0.5 mM) oxidation by H2O2 (0.1 mM) mediated by LPO previously incubated in the absence (upper trace) or in the presence of different GSH concentrations (remaining traces). LPO was 5 μM before dilution into the spectrophotometer’s cuvette. Reactions of LPO and GSH were stopped by NEM addition to the incubations at a final 20 mM concentration. Experiments were performed in triplicate. LPO was preincubated with the following concentrations of GSH: 0.15 mM; 0.5 mM, and 2 mM. Alternatively, GSH (10 – 100 μM) was added as indicated.