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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2007 Jan 8;42(7):985–992. doi: 10.1016/j.freeradbiomed.2006.12.026

Fig. 8.

Fig. 8

Immunochemical detection of the DMPO/GS-nitrone adduct by ELISA. LPO (5 μM) was incubated with GSH (5 mM) and DMPO (100 mM) for 2h at 37 °C. After samples were filtered through Millipore ultrafree mass cut filters (30 kDa), both low and high molecular weight fractions were analyzed through ELISA. Protein fractions were re-suspended and washed twice with phosphate buffer prior to transferring to the ELISA plate. DMPO-GSH standards were also incubated on the ELISA plates at the concentrations indicated on the figure. Each column is the average of three independent experiments.