Figure 1. Spontaneous Odor Intensity Discrimination Does Not Require the Normal Function of MB.

In this and the following figures, all control groups are shown in black bars, and the experimental groups in white bars, or blue when statistically significantly different from the control (p < 0.05), and all experiments were done in a balanced and experimenter-blind manner.

Spontaneous intensity discrimination between different concentrations of MCH (left panels) or BA (right panels) was not affected (A) by MB ablation (+HU), (B) by transient block (30 °C) of temperature-sensitive (20 °C, permissive; 30 °C, restrictive) shibire-dependent synaptic transmission using three different MB PGAL4 drivers (C309, 247, and 201Y), (C) by RNAi-mediated disruption of G_αq using the same MB PGAL4 drivers, or (D) by overexpression of constitutively active G_αs* (with G_αs ⁺ as controls) using two MB PGAL4 drivers (C309 and 247). n = 4 PIs for all groups.