(A−E) Spontaneous odor identity discrimination between MCH and BA was severely disrupted (A) by MB ablation (+HU), (B) by transient block (30 °C) of synaptic transmission from MB in C309/shits, 247/shits, or 201Y/shits flies, (C) by developmental disruption of Gαq in MB of dsGαq/C309, dsGαq/247, and dsGαq/201Y flies, (D) by adult-specific, physiological disruption of Gαq in MB of dsGαq, GAL80ts/C309 flies at restrictive (30 °C for 3 d) but not permissive (18 °C for 3 d) temperature, or (E) by developmental disruption of Gαq in MB of dGq
1F1/C309 or dGq
1F1/247 flies.
(F−H) Spontaneous odor identity discrimination was not affected (F) when the drl gene was knocked down in MB via RNAi-mediated disruption (T. T., H. Tang, and M. Regulski, unpublished data) in drl
RNAi/C309 or drl
RNAi/247 flies, (G) when Gαs was disrupted in MB of Gαs*/C309, Gαs*/247, or Gαs*/201Y flies, or (H) in rut and dnc mutants. n = 4 PIs for all groups.