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GRP cells exhibit extensive self-renewal potential. A2B5+ GRP cells were immunopurified from E13.5 spinal cord and expanded in PDGF + bFGF. Each of the five randomly selected primary clones gave rise to from 66 to 260 secondary clones, and each of the randomly selected secondary clones gave rise to 2–132 tertiary clones. An example of an expanded clone is shown as a phase image and labeled as living cells with A2B5 antibody. (Bar = 40 μm for days 1 and 3; 400 μm for day 10).
