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. Author manuscript; available in PMC: 2007 Sep 28.
Published in final edited form as: Anal Biochem. 2006 Dec 20;363(1):12–21. doi: 10.1016/j.ab.2006.10.041

Fig. 3.

Fig. 3

Comparison of rates of hydrolysis of different substrates by FAAH. Assays were performed at 37 °C in 200 μL 0.1 M, pH 7.4, sodium phosphate buffer (A) or 0.1 M, pH 9.0, Tris–HCl buffer (B) containing 50 μM substrate, 1% glycerol, 0.1% Triton X-100, 3% DMSO, and microsomal protein (0.67 μg for Octanoyl-MP, Octanoyl-MMP, and Arachdonyl-MMP and 100 μg for Octanoyl-TFMCoumarin). The assays were recorded by a 2-min interval during 90 min. The excitation and emission wavelengths (nm) for Octanoyl-MP, Octanoyl-MMP, and Arachidonyl-MMP were 302 and 396, 304 and 392, and 302 and 396, respectively. Similarly, 366 and 496 were excitation and emission wavelengths (nm) for Octanoyl-TFMCoumarin.