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Expression of tight junction mRNA with increasing passage number
| P25 | P30 | P35 | P40 | |
|---|---|---|---|---|
| ZO-1 | + | + | − | + |
| ZO-2 | + | + | + | + |
| Occludin | + | + | + | + |
| Claudin-1 | − | − | − | − |
| Claudin-3 | − | − | − | − |
| Claudin-5 | + | + | + | + |
| Actin | + | + | + | + |
| GAPDH | + | + | + | + |
Presence or absence of ethidium bromide detection of tight junction mRNA at each passage number is indicated by + or −. Reactions amplifying GAPDH as a control for starting mRNA concentrations were run for all samples. RT-PCR experiments were repeated 3–4 times.
