Table 1.

Error-prone PCR nucleotide mutation spectrum. The spectrum was determined by sequencing the four final (generation 12) sequences from the unselected population, as in these sequences the mutations accumulate without constraint. As has been previously noted for error-prone PCR with Taq polymerase [3,5,27], the nucleotide error spectrum is biased towards certain types of mutations.

Parameter	Value
Total nucleotide mutations	67
% Synonymous mutations	25
Mutation types (%)
A → T, T → A	19.4
A → C, T → G	1.5
A → G, T → C	64.2
G → A, C → T	4.5
G → C, C → G	0.0
G → T, C → A	1.5
Frameshift	9.0