Table 2.

List of primers used for reverse transcriptase-polymer chain reactions, site-directed deletion mutagenesis, and ribonuclease protection assay

Gene			Sequence
RT-PCR
TβRI	P1	S:	5'-CGAGGTTTGCTGGGGTGAGGCA-3'
		AS:	5'-CTCAGTGAGGTAGAACAACTGACC-3'
	P2	S:	5'-AACAAGCTTTCCACCATGGAGGCGGCGGT-3'
		AS:	5'-GTTCATATCTTACATTTTGATGCCTTCCTGT-3'
β-Actin		S:	5'-CCCTTTTTGTCCCCCAAC-3'
		AS:	5'-CTGGTCTCAAGTCAGTGTACAGGT-3'
TβRI-HA	P3	S:	5'-ATCTATGCAATGGGCTT-3'
		AS:	5'-AGCGTAATCTGGAACATCGTA-3'
Mutagenesis
TβRI	DS1	S:	5'-ATGGAGGCGGCGGTCGCGGCGGCGGCGGCGGCG-3'
		AS:	5'-CGCCGCCGCCGCCGCCGCGACCGCCGCCTCCAT-3'
	DS2	S:	5'-CTGCTCCTCCTCGTGCTGCTGCTCCCGGGGGCGACG-3'
		AS:	5'-CGTCGCCCCCGGGAGCAGCAGCACGAGGAGGAGCAG-3'
	DS3	S:	5'-GCGGCGGCGGCGGCGGCGCTCTGTACAAAAGACAAT-3'
		AS:	5'-ATTGTCTTTTGTACAGAGCGCCGCCGCCGCCGCCGC-3'
RPA
TβRI	DM1	S:	5'-ACCATGGAGGCGGCGGT-3'
		AS:	5'-TAATACGACTCACTATAGGGACCACCACCTCCTTATTGC-AATGGTCCTGA-3'
	DM2	S:	5'-ACCATGGAGGCGGCGGT-3'
		AS:	5'-TAATACGACTCACTATAGGGACCACCACCTCCAGAGGGT-GCACATACAA-3'
	DM3	S:	5'-AACAAGCTTTCCACCATGGAGGCGGCGGTCGC-3'
		AS:	5'-TAATACGACTCACTATAGGGACCACCACCTCCTTCAGCT-ATACACATGCT-3'
	DM4	S:	5'-AACAAGCTTTCCACCATGGAGGCGGCGGTCGC-3'
		AS:	5'-TAATACGACTCACTATAGGGACCACCACCTCCATCTCTA-TGAGCAATGGCT-3'

S, sense primer; AS, antisense primer; RPA, ribonuclease protection assay